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Free download Biological Safety Manual ebook

Wednesday, July 16, 2008

Free download Biological Safety Manual ebook. The purpose of this manual is to set biological safety policies. This manual contains information, regulations and procedures to provide for the safety of personnel and the environment from biologically hazardous materials. This manual is free online!

Table of Contents:

1 — Biological Safety

Principles of Biological Safety

Laboratory Practice and Technique
Safety Equipment (Primary Barrier)
Facility Design (Secondary Barrier)
Biosafety Levels
Animal Facilities
Clinical Laboratories
Importation and Interstate Shipment of Certain Biomedical Materials

Biological Safety Levels

2 — Information for Researchers

Project Registration

Bio-Agent (BA) Registration
Recombinant DNA (R-DNA) Registration
Acute Toxins (AT) Registration
Regulated Biological Materials
Table 3: Toxin Table
Select Agents
Select Agents List
Minors in Research Laboratories or Animal Facilities

Biological Waste Disposal Policy

Categories
1) Infectious/potentially infectious/R-DNA
2) Non-infectious waste
3) Mixed radioactive/biohazardous waste
4) Mixed chemical/biohazardous waste
5) Animal carcasses and materials
6) Human remains
Packaging
1) Biohazard bags
2) Sharps
3) Corrugated cardboard boxes
Labeling

Biological Waste Disposal Containers

Biohazard Boxes
Sharps Boxes
Biohazard Bags

Autoclave Use and Testing

Record-Keeping

Disinfectants

Liquids
Alcohols,Chlorine compounds,Formaldehyde,
Glutaraldehyde,Hydrogen peroxide, Iodine and Iodophors,
Phenol and phenolic compounds, Quaternary ammonium compounds

Gases
Ethylene Oxide, Vapor Phase Hydrogen Peroxide,
Chlorine Dioxide gas, Ozone, Formaldehyde Gas (from heating paraformaldehyde)

Irradiation
Ultraviolet, UV radiation, Ionizing radiation, Electron Beam, Microwaves,

Disinfectants Bibliography

Shipment of Biological Materials

Biological materials subject to shipping & transport regulations:
Transporting biological material within and around UF:
Permits:
Biological Safety Cabinets

Biological Safety Cabinets (BSCs)

Horizontal Laminar Flow “Clean Bench”
Vertical Laminar Flow “Clean Bench”
Operations within a Class II BSC
Laboratory Hazards
Decontamination
Surface Decontamination
Gas Decontamination
Engineering Requirements
Ultraviolet Lamps
BSC Placement
HEPA Filters
Certification of BSCs

Emergency Procedures/Telephone Numbers

3 — Programs
Animal Contact Medical Monitoring Program
UF Bloodborne Pathogen Program
BBP Exposure Information

4 — Medical Surveillance
HIV Research Laboratory Occupational Medicine Policy
Pre-employment
Continuing employment
Post-exposure prophylaxis
References
Immunoprophylaxis
Recommendations for Prophylactic Immunization of Laboratory Personnel Working
with Infectious Agents
Bacterial agents
Rickettsial agents
Viral agents
Vaccinia Immunization Policy
University of Florida
Health Surveillance for Personnel Working with Infectious Agents
Blood Serum Sampling
Health Assessments
Exposure to Mycobacterium Tuberculosis

5 — Appendix A – Forms
R-DNA
Bio-Agent
Transgenic Animals
Acute Toxin
Project Addendum

Download free Biological Safety Manual ebook in PDF.

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